Coding

Part:BBa_K3595007:Design

Designed by: Ying Li   Group: iGEM20_GZ_HFI   (2020-10-26)


Mutant cysE-11-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 684
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Substituting CGT (Arg), ACC (Thr) in 89th, 90th positions into CAT (His), GTA (Val)), and substituting CCG (Pro), GCA (Ala) in 93th and 94th position into GCT (Ala), ACA (Thr) of the wild-type cysE sequence.


Source

Synthetic

References